Data/hora: 18/05/2024 06:14:47

Biblioteca(s): 	Embrapa Agroindústria Tropical.	
Data corrente: 	01/06/1994	
Data da última atualização: 	01/06/1994	
Autoria: 	BURGER, D. W.	
Título: 	Guidelines for autoclaving liquid media used in plant tissue culture.	
Ano de publicação: 	1988	
Fonte/Imprenta: 	Horscience, v.23, n.6, p.1066-1068, 1988.	
Idioma: 	Inglês	
Conteúdo: 	The temperatures of liquid volumes (10-4000 ml) were monitored with solid-state temperature probes during autoclaving cycles of an automated steam sterilizer. Accurate measurements of the time required for the liquid volumes to reach the sterilizing temperature (121oC) were made. The effects of preheating solutions to be autoclaved and enclosing materials in autoclavable plastic bags were determined. Liquid volumes between 10 and 4000 ml differed in the time required to reach 121oC by 40 min. Preheating solutions before autoclaving slightly reduced the time required to reach sterilizing temperatures. Enclosing materials in autoclavable bags substantially increased the time required to reach 121o.	
Palavras-Chave: 	Autoclavagem; Cultura de tecidos.	
Thesagro: 	Esterilização; Meio de Cultura.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroindústria Tropical (CNPAT)	
Exemplares: 	CNPAT - (1047-1) - EMB - SP - -- - SP 01415 -  - 94.01415 - DIS